The majority of RNA molecules are tRNAs and rRNAs. mRNA accounts for only 1–5% of the total cellular RNA although the actual amount depends on the cell type and physiological state. Approximately 360,000 mRNA molecules are present in a single mammalian cell, made up of approximately 12,000 different transcripts with a typical length of ...

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Total RNA binds to the RNeasy membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in RNase-free water. Using the mechanical disruption method, yeast cells are lysed and homogenized by high-speed agitation in the TissueLyser or other bead mill in the presence of glass beads and Buffer RLT.

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Total RNA was isolated from budwood obtained from infected apple (1), cherry (2), pear (3), and grapevine (4–8), leaf tissue from black currant (9, 10), and dormant tuber tissue from infected potato (11, 12) using a modification of the RNeasy protocol.Viral RNA was amplified using a one-tube RT-PCR. Amplification products were analyzed on a 1.5% agarose gel.

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p73 regulates epidermal wound healing and induced keratinocyte programming. Beeler JS, Marshall CB, Gonzalez-Ericsson PI, Shaver TM, Santos Guasch GL, Lea ST, Johnson KN, Jin H, Venters BJ, Sanders ME, Pietenpol JA p63 is a transcriptional regulator of ectodermal development that is required for basal cell proliferation and stem cell maintenance. p73 is a closely related p53 family member that ...

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